An improved diacetyl reaction for the estimation of urea in blood.
نویسنده
چکیده
منابع مشابه
A new colorimetric method for the determination of urea nitrogen in blood with diacetyl monoxime-glucuronolactone-glucosaccharodilactone reagent.
In a previous paper,3) a colorimetric method for the determination of urea nitrogen in blood and urine was presented on the basis of a color reaction of urea with diacetyl monoxime (DAM) in phosphoric acid solution in the presence of D-glucuronolactone (GL), which served to produce a photo-stable color and eliminate the sigmoidal nature of calibration curve observed usually in the DAM method.4)...
متن کاملDetermination of Urea in Blood and Urine with Diacetyl Monoxime-glucuronolactone Reagent.
N UMEROUS STUDIES have been reported in the literature on the photometric determination of urea in biological fluids with diacetyl monoxime as the color-developing agent. The reaction is photosensitive, does not follow Beer’s law, and requires special precautions for reproducible results. In many proposed procedures, oxidants were added in the reaction mixture to destroy hydroxylamine which mig...
متن کاملA note on urea estimation by diacetyl monoxime.
Rosenthal (1955) has described in great detail a method using diacetyl monoxime which is generally satisfactory for the routine estimation of urea in blood and urine except that complete colour development requires prolonged heating in boiling water. This disadvantage can be overcome by the use of a pressure cooker. The tubes of reaction mixture are immersed in a beaker filled with hot water to...
متن کاملDirect fluorometric determination of urea in urine.
Urea in biological fluids has been determined by a variety of direct and indirect procedures. Some indirect methods depend on the hydrolysis of urea with the enzyme urease to form ammonia, with subsequent quantitation of the ammonia by nesslerization (1-4). Undesirable features of these indirect methods are the use of relatively unstable reagents and loss of ammonia during the procedure. Direct...
متن کاملArtifactually elevated BUN values on the Boehringer Mannheim-Hitachi 737 and 705 automated chemistry analysers and the development of a kinetic BUN method
The measurement of blood urea nitrogen (BUN) as serum or plasma urea is one of the most frequently ordered laboratory tests for evaluating renal functions. Conventional analysis of reactions of urea with diacetyl monoxime or urease with the Berthelot reaction [1] is rapidly giving way to the totally enzymatic reactions that are more convenient to perform and more easily adapted to modern instru...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 42 3 شماره
صفحات -
تاریخ انتشار 1948